variable capacitance differential pressure transducer type 270b electronic display unit Search Results


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Miltenyi Biotec cd22
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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MKS Instruments variable capacitance differential pressure transducer type 270b electronic display unit
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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Mitsubishi Rayon CO kpf 270b
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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Multigate Medical Products Pty Ltd dielectric fin 270b
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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Mitutoyo mitutoyo 543 270b dial indicator
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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BioMimetic Therapeutics valve unit 270
Immunophenotyping panel for multiplexed tissue imaging of cancer.
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Lonza human mononuclear cells (hmncs
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
Human Mononuclear Cells (Hmncs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ciba Geigy cgs 27023a
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
Cgs 27023a, supplied by Ciba Geigy, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SCHOTT b-270
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
B 270, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Novartis mmi270
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
Mmi270, supplied by Novartis, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iCell Gene Therapeutics isled mibs master cell 270
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
Isled Mibs Master Cell 270, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MKS Instruments differential manometer system mks baratron model 698a
Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses <t>using</t> <t>hPBMCs.</t> The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated <t>hMNCs</t> were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
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Image Search Results


Immunophenotyping panel for multiplexed tissue imaging of cancer.

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD22 , REA340 , 50 , 130-124-223 , FITC , Miltenyi Biotec.

Techniques: Imaging

Deep spatial profiling of human palatine tonsil tissues. (A) Hematoxylin and eosin (H&E) staining after 92 MICS cycles, including the marked epithelium, germinal center (GC), and T cell zone of the lymphoid follicle. MICS DAPI and stroma staining depicting the composition and structure of the tonsil. Markers: Collagen III, collagen IV, fibronectin (all extracellular matrix (ECM), cytokeratin (epithelium), podoplanin (lymphatic vessels), CD105/SM Actin (blood vessels). (B) Immune cell content of a human palatine tonsil comprising T cells (CD3), B cells (CD19/CD20), plasma cells (PCs) (CD38/CD138), NK cells (CD56), granulocytes (CD15/CD66b), mast cells (CD117), macrophages (MΦ) (CD163/CD169/CD206), myeloid dendritic cells (mDCs) (CD11c), and plasmacytoid dendritic cells (pDCs) (CD123). (C) Detailed view on the T cell zone, mainly composed of CD4 + helper T cells (T h ) and CD8 + cytotoxic T cells (T c ), mDCs (CD11c), and PCs (CD38/CD138). (D) Detailed view on the GC-mantle zone border, showing different B cells (CD11b, CD21, CD22), mDCs (CD11c), and PCs (CD38/CD138). (E) Cell annotations of three different tonsil samples plus respective bar graphs of gated cell populations, comparing the cell content between the three tonsil samples. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: 976 x 640 µm, zoomed-in subregions in (C, D) : 334 µm x 219 µm. Scale bar: 100 µm.

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Deep spatial profiling of human palatine tonsil tissues. (A) Hematoxylin and eosin (H&E) staining after 92 MICS cycles, including the marked epithelium, germinal center (GC), and T cell zone of the lymphoid follicle. MICS DAPI and stroma staining depicting the composition and structure of the tonsil. Markers: Collagen III, collagen IV, fibronectin (all extracellular matrix (ECM), cytokeratin (epithelium), podoplanin (lymphatic vessels), CD105/SM Actin (blood vessels). (B) Immune cell content of a human palatine tonsil comprising T cells (CD3), B cells (CD19/CD20), plasma cells (PCs) (CD38/CD138), NK cells (CD56), granulocytes (CD15/CD66b), mast cells (CD117), macrophages (MΦ) (CD163/CD169/CD206), myeloid dendritic cells (mDCs) (CD11c), and plasmacytoid dendritic cells (pDCs) (CD123). (C) Detailed view on the T cell zone, mainly composed of CD4 + helper T cells (T h ) and CD8 + cytotoxic T cells (T c ), mDCs (CD11c), and PCs (CD38/CD138). (D) Detailed view on the GC-mantle zone border, showing different B cells (CD11b, CD21, CD22), mDCs (CD11c), and PCs (CD38/CD138). (E) Cell annotations of three different tonsil samples plus respective bar graphs of gated cell populations, comparing the cell content between the three tonsil samples. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: 976 x 640 µm, zoomed-in subregions in (C, D) : 334 µm x 219 µm. Scale bar: 100 µm.

Article Snippet: CD22 , REA340 , 50 , 130-124-223 , FITC , Miltenyi Biotec.

Techniques: Staining, Clinical Proteomics

Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses using hPBMCs. The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated hMNCs were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.

Journal: EBioMedicine

Article Title: A peptide derived from the core β-sheet region of TIRAP decoys TLR4 and reduces inflammatory and autoimmune symptoms in murine models

doi: 10.1016/j.ebiom.2020.102645

Figure Lengend Snippet: Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses using hPBMCs. The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated hMNCs were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.

Article Snippet: Human peripheral blood mononuclear cells (hPBMCs) and human mononuclear cells (hMNCs) were purchased from Lonza Inc. (Allendale, NJ, USA) and cultured in RPMI 1640 containing 2·05 mM l -glutamine, 1% of the penicillin/streptomycin solution, and 10% of FBS.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test