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Lonza
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iCell Gene Therapeutics
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MKS Instruments
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
doi: 10.3389/fimmu.2024.1383932
Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.
Article Snippet:
Techniques: Imaging
Journal: Frontiers in Immunology
Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging
doi: 10.3389/fimmu.2024.1383932
Figure Lengend Snippet: Deep spatial profiling of human palatine tonsil tissues. (A) Hematoxylin and eosin (H&E) staining after 92 MICS cycles, including the marked epithelium, germinal center (GC), and T cell zone of the lymphoid follicle. MICS DAPI and stroma staining depicting the composition and structure of the tonsil. Markers: Collagen III, collagen IV, fibronectin (all extracellular matrix (ECM), cytokeratin (epithelium), podoplanin (lymphatic vessels), CD105/SM Actin (blood vessels). (B) Immune cell content of a human palatine tonsil comprising T cells (CD3), B cells (CD19/CD20), plasma cells (PCs) (CD38/CD138), NK cells (CD56), granulocytes (CD15/CD66b), mast cells (CD117), macrophages (MΦ) (CD163/CD169/CD206), myeloid dendritic cells (mDCs) (CD11c), and plasmacytoid dendritic cells (pDCs) (CD123). (C) Detailed view on the T cell zone, mainly composed of CD4 + helper T cells (T h ) and CD8 + cytotoxic T cells (T c ), mDCs (CD11c), and PCs (CD38/CD138). (D) Detailed view on the GC-mantle zone border, showing different B cells (CD11b, CD21, CD22), mDCs (CD11c), and PCs (CD38/CD138). (E) Cell annotations of three different tonsil samples plus respective bar graphs of gated cell populations, comparing the cell content between the three tonsil samples. Depicted markers and annotated cell types as indicated by the color code. ROI sizes: 976 x 640 µm, zoomed-in subregions in (C, D) : 334 µm x 219 µm. Scale bar: 100 µm.
Article Snippet:
Techniques: Staining, Clinical Proteomics
Journal: EBioMedicine
Article Title: A peptide derived from the core β-sheet region of TIRAP decoys TLR4 and reduces inflammatory and autoimmune symptoms in murine models
doi: 10.1016/j.ebiom.2020.102645
Figure Lengend Snippet: Characterisation of the TLR-antagonistic effects of TIP3 on primary cells. (a) TIP3 was found to be safe for THP-1 cells at multiple concentrations (≤ 50 μM) for 24 h. (b, c) The protein expression was measured by western blot analyses using hPBMCs. The amounts of p-p65, Iκ-Bα, p-IRF3, ATF3, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 were evaluated in the total-protein extract. β-Actin served as a loading control. (d, e) The secretion levels of TNF-α and IL-6 were measured by ELISA after the cells were stimulated with LPS and then treated with TIP3. (f–i) The effects of TIP3 on the LPS-induced mRNA expression levels of IL-6, TNF-α, IL-8, and IL-1β after 4 h. The mRNA levels of these genes were substantially downregulated by TIP3. Values are indicated as fold changes (relative quantification; RQ) in mRNA levels, normalized to GAPDH. (j–m) The LPS-stimulated mBMDMs were treated with TIP3 for 1 h and the secretion levels of (j) IL-6 and (k) TNF-α were measured by an ELISA, and (m) the production of NO was evaluated using NO secretion kit. (l) The, IFN-β level was measured in poly(I:C)-stimulated mBMDMs through ELISA. (n-p) The LPS-stimulated hMNCs were also utilized to evaluate the secretion levels of (o) IL-6 and (p) TNF-α by ELISA. (q) The poly (I:C)-stimulated hMNCs were employed to assess the TLR3-inhibitory effect of TIP3 by measuring the TNF-α secretion by an ELISA. Effects similar to those of in RAW264.7 cells were detected. The data shown represent at least three independent experiments ( n ≥ 3), and bars denote mean ± SEM (* P < 0·05, ** P < 0·01) according to two-tailed Student's t -test.
Article Snippet: Human peripheral blood mononuclear cells (hPBMCs) and
Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test